Isolation Of Entomopathogenic Nematodes From Soil By The “Galleria Trap” Method And Their Efficacy Against The Cotton Bollworm (Helicoverpa Armigera)
Keywords:
Entomopathogenic nematodes, Steinernema, Heterorhabditis, Galleria mellonellaAbstract
Entomopathogenic nematodes were isolated by the Galleria trap method from 60 soil samples collected in Urtachirchik and Yukorichirchik districts of Tashkent region, and their efficacy against cotton bollworm (Helicoverpa armigera Hbn.) larvae was assessed. 20.0% of samples were positive (9 Steinernema, 3 Heterorhabditis isolates). Steinernema sp. OCh-2 at 100 IJs/larva caused 92.5% mortality after 72 h (LC₅₀ = 20.4 IJs/larva).
References
Kaya H.K., Gaugler R. Entomopathogenic nematodes // Annual Review of Entomology. – 1993. – Vol. 38. – P. 181–206.
Bedding R.A., Akhurst R.J. A simple technique for the detection of insect parasitic rhabditid nematodes in soil // Nematologica. – 1975. – Vol. 21. – P. 109–110.
White G.F. A method for obtaining infective nematode larvae from cultures // Science. – 1927. – Vol. 66. – P. 302–303.
Abbott W.S. A method of computing the effectiveness of an insecticide // Journal of Economic Entomology. – 1925. – Vol. 18. – P. 265–267.
Grewal P.S., Ehlers R.-U., Shapiro-Ilan D.I. (eds.) Nematodes as Biocontrol Agents. – Wallingford: CABI Publishing, 2005. – 505 p.
Shapiro-Ilan D.I., Hazir S., Glazer I. Basic and applied research: entomopathogenic nematodes // Microbial Control of Insect and Mite Pests / ed. L.A. Lacey. – Academic Press, 2017. – P. 91–105.
Kaya H.K., Stock S.P. Techniques in insect nematology // Manual of Techniques in Insect Pathology / ed. L.A. Lacey. – San Diego: Academic Press, 1997. – P. 281–324.
Hominick W.M. Biogeography // Entomopathogenic Nematology / ed. R. Gaugler. – Wallingford: CABI Publishing, 2002. – P. 115–143.
Lacey L.A., Georgis R. Entomopathogenic nematodes for control of insect pests above and below ground with comments on commercial production // Journal of Nematology. – 2012. – Vol. 44, No. 2. – P. 218–225.
